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dc.contributor.authorK. Chu, Kengyehen_US
dc.contributor.authorLim, Darylen_US
dc.contributor.authorMertz, Jeromeen_US
dc.date.accessioned2012-01-11T00:39:11Z
dc.date.available2012-01-11T00:39:11Z
dc.date.copyright2010en_US
dc.date.issued2010-7-16en_US
dc.identifier.citationK. Chu, Kengyeh, Daryl Lim, Jerome Mertz. "Practical implementation of log-scale active illumination microscopy" Biomedical Optics Express 1(1): 236-245. (2010)en_US
dc.identifier.issn2156-7085en_US
dc.identifier.urihttp://hdl.handle.net/2144/3012
dc.description.abstractActive illumination microscopy (AIM) is a method of redistributing dynamic range in a scanning microscope using real-time feedback to control illumination power on a sub-pixel time scale. We describe and demonstrate a fully integrated instrument that performs both feedback and image reconstruction. The image is reconstructed on a logarithmic scale to accommodate the dynamic range benefits of AIM in a single output channel. A theoretical and computational analysis of the influence of noise on active illumination feedback is presented, along with imaging examples illustrating the benefits of AIM. While AIM is applicable to any type of scanning microscope, we apply it here specifically to two-photon microscopy.en_US
dc.description.sponsorshipNational Institutes of Healthen_US
dc.language.isoenen_US
dc.publisherOptical Society of Americaen_US
dc.rightsCopyright 2010 Optical Society of America This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en_US
dc.subject(110.0180) Microscopyen_US
dc.subject(180.2520) Fluorescence microscopyen_US
dc.subject(180.5810) Scanning microscopyen_US
dc.subject(190.4180) Multiphoton processesen_US
dc.titlePractical Implementation of Log-Scale Active Illumination Microscopyen_US
dc.typearticleen_US
dc.identifier.doi10.1364/BOE.1.000236en_US
dc.identifier.pmcid3005158en_US


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Copyright 2010 Optical Society of America This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
Except where otherwise noted, this item's license is described as Copyright 2010 Optical Society of America This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.