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dc.contributor.authorBoyd, Ashleigh S.en_US
dc.contributor.authorWood, Kathryn J.en_US
dc.date.accessioned2012-01-12T17:35:46Z
dc.date.available2012-01-12T17:35:46Z
dc.date.issued2010-6-4en_US
dc.identifier.citationBoyd, Ashleigh S., Kathryn J. Wood. "Characteristics of the Early Immune Response Following Transplantation of Mouse ES Cell Derived Insulin-Producing Cell Clusters" PLoS ONE 5(6): e10965. (2010)en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/2144/3405
dc.description.abstractBACKGROUND. The fully differentiated progeny of ES cells (ESC) may eventually be used for cell replacement therapy (CRT). However, elements of the innate immune system may contribute to damage or destruction of these tissues when transplanted. METHODOLOGY/PRINCIPAL FINDINGS. Herein, we assessed the hitherto ill-defined contribution of the early innate immune response in CRT after transplantation of either ESC derived insulin producing cell clusters (IPCCs) or adult pancreatic islets. Ingress of neutrophil or macrophage cells was noted immediately at the site of IPCC transplantation, but this infiltration was attenuated by day three. Gene profiling identified specific inflammatory cytokines and chemokines that were either absent or sharply reduced by three days after IPCC transplantation. Thus, IPCC transplantation provoked less of an early immune response than pancreatic islet transplantation. CONCLUSIONS/SIGNIFICANCE. Our study offers insights into the characteristics of the immune response of an ESC derived tissue in the incipient stages following transplantation and suggests potential strategies to inhibit cell damage to ensure their long-term perpetuation and functionality in CRT.en_US
dc.description.sponsorshipMedical Research Council; BD Preanalytical Systems Europe; National Institutes of Health (P20RR018757); Roger Williams Medical Center; Wellcome Trust; European Union through the Optistem Network; Royal Society (Wolfson Research Merit Award)en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsBoyd, Wood. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleCharacteristics of the Early Immune Response Following Transplantation of Mouse ES Cell Derived Insulin-Producing Cell Clustersen_US
dc.typearticleen_US
dc.identifier.doi10.1371/journal.pone.0010965en_US
dc.identifier.pubmedid20532031en_US
dc.identifier.pmcid2881030en_US


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