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dc.contributor.authorYu, Yingpuen_US
dc.contributor.authorMarintchev, Assenen_US
dc.contributor.authorKolupaeva, Victoria G.en_US
dc.contributor.authorUnbehaun, Anetten_US
dc.contributor.authorVeryasova, Tatyanaen_US
dc.contributor.authorLai, Shao-Chiangen_US
dc.contributor.authorHong, Pengen_US
dc.contributor.authorWagner, Gerharden_US
dc.contributor.authorHellen, Christopher U. T.en_US
dc.contributor.authorPestova, Tatyana V.en_US
dc.date.accessioned2012-01-12T17:34:26Z
dc.date.available2012-01-12T17:34:26Z
dc.date.copyright2009en_US
dc.date.issued2009-8en_US
dc.identifier.citationYu, Yingpu, Assen Marintchev, Victoria G. Kolupaeva, Anett Unbehaun, Tatyana Veryasova, Shao-Chiang Lai, Peng Hong, Gerhard Wagner, Christopher U. T. Hellen, Tatyana V. Pestova. "Position of eukaryotic translation initiation factor eIF1A on the 40S ribosomal subunit mapped by directed hydroxyl radical probing" Nucleic Acids Research 37(15): 5167-5182. (2009)en_US
dc.identifier.issn1362-4962en_US
dc.identifier.urihttp://hdl.handle.net/2144/3399
dc.description.abstractThe universally conserved eukaryotic initiation factor (eIF), eIF1A, plays multiple roles throughout initiation: it stimulates eIF2/GTP/Met-tRNAiMet attachment to 40S ribosomal subunits, scanning, start codon selection and subunit joining. Its bacterial ortholog IF1 consists of an oligonucleotide/oligosaccharide-binding (OB) domain, whereas eIF1A additionally contains a helical subdomain, N-terminal tail (NTT) and C-terminal tail (CTT). The NTT and CTT both enhance ribosomal recruitment of eIF2/GTP/Met-tRNAiMet, but have opposite effects on the stringency of start codon selection: the CTT increases, whereas the NTT decreases it. Here, we determined the position of eIF1A on the 40S subunit by directed hydroxyl radical cleavage. eIF1A's OB domain binds in the A site, similar to IF1, whereas the helical subdomain contacts the head, forming a bridge over the mRNA channel. The NTT and CTT both thread under Met-tRNAiMet reaching into the P-site. The NTT threads closer to the mRNA channel. In the proposed model, the NTT does not clash with either mRNA or Met-tRNAiMet, consistent with its suggested role in promoting the 'closed' conformation of ribosomal complexes upon start codon recognition. In contrast, eIF1A-CTT appears to interfere with the P-site tRNA-head interaction in the 'closed' complex and is likely ejected from the P-site upon start codon recognition.en_US
dc.description.sponsorshipNational Institutes of Health (GM59660, GM47467, CA68268); National Cancer Institute (Howard Temin award K01 CA119107); Boston University (Peter Paul Career Development Professorship award)en_US
dc.language.isoenen_US
dc.rightsCopyright 2009 Yu, Yingpu, Assen Marintchev, Victoria G. Kolupaeva, Anett Unbehaun, Tatyana Veryasova, Shao-Chiang Lai, Peng Hong, Gerhard Wagner, Christopher U. T. Hellen, Tatyana V. Pestovaen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.0/uk/en_US
dc.titlePosition of Eukaryotic Translation Initiation Factor eIF1A on the 40S Ribosomal Subunit Mapped by Directed Hydroxyl Radical Probingen_US
dc.typearticleen_US
dc.identifier.doi10.1093/nar/gkp519en_US
dc.identifier.pubmedid19561193en_US
dc.identifier.pmcid2731904en_US


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Copyright 2009 Yu, Yingpu, Assen Marintchev, Victoria G. Kolupaeva, Anett Unbehaun, Tatyana Veryasova, Shao-Chiang Lai, Peng Hong, Gerhard Wagner, Christopher U. T. Hellen, Tatyana V. Pestova
Except where otherwise noted, this item's license is described as Copyright 2009 Yu, Yingpu, Assen Marintchev, Victoria G. Kolupaeva, Anett Unbehaun, Tatyana Veryasova, Shao-Chiang Lai, Peng Hong, Gerhard Wagner, Christopher U. T. Hellen, Tatyana V. Pestova