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dc.contributor.authorVaickus, Louis Jen_US
dc.contributor.authorBouchard, Jacquelineen_US
dc.contributor.authorKim, Jiyounen_US
dc.contributor.authorNatarajan, Sudhaen_US
dc.contributor.authorRemick, Daniel Gen_US
dc.date.accessioned2012-01-11T23:19:34Z
dc.date.available2012-01-11T23:19:34Z
dc.date.copyright2010en_US
dc.date.issued2010-11-23en_US
dc.identifier.citationVaickus, Louis J, Jacqueline Bouchard, Jiyoun Kim, Sudha Natarajan, Daniel G Remick. "Oral tolerance inhibits pulmonary eosinophilia in a cockroach allergen induced model of asthma: a randomized laboratory study" Respiratory Research 11(1):160. (2010)en_US
dc.identifier.issn1465-993Xen_US
dc.identifier.urihttp://hdl.handle.net/2144/3340
dc.description.abstractBACKGROUND. Antigen desensitization through oral tolerance is becoming an increasingly attractive treatment option for allergic diseases. However, the mechanism(s) by which tolerization is achieved remain poorly defined. In this study we endeavored to induce oral tolerance to cockroach allergen (CRA: a complex mixture of insect components) in order to ameliorate asthma-like, allergic pulmonary inflammation. METHODS. We compared the pulmonary inflammation of mice which had received four CRA feedings prior to intratracheal allergen sensitization and challenge to mice fed PBS on the same time course. Respiratory parameters were assessed by whole body unrestrained plethysmography and mechanical ventilation with forced oscillation. Bronchoalveolar lavage fluid (BAL) and lung homogenate (LH) were assessed for cytokines and chemokines by ELISA. BAL inflammatory cells were also collected and examined by light microscopy. RESULTS. CRA feeding prior to allergen sensitization and challenge led to a significant improvement in respiratory health. Airways hyperreactivity measured indirectly via enhanced pause (Penh) was meaningfully reduced in the CRA-fed mice compared to the PBS fed mice (2.3 ± 0.4 vs 3.9 ± 0.6; p = 0.03). Directly measured airways resistance confirmed this trend when comparing the CRA-fed to the PBS-fed animals (2.97 ± 0.98 vs 4.95 ± 1.41). This effect was not due to reduced traditional inflammatory cell chemotactic factors, Th2 or other cytokines and chemokines. The mechanism of improved respiratory health in the tolerized mice was due to significantly reduced eosinophil numbers in the bronchoalveolar lavage fluid (43300 ± 11445 vs 158786 ± 38908; p = 0.007) and eosinophil specific peroxidase activity in the lung homogenate (0.59 ± 0.13 vs 1.19 ± 0.19; p = 0.017). The decreased eosinophilia was likely the result of increased IL-10 in the lung homogenate of the tolerized mice (6320 ± 354 ng/mL vs 5190 ± 404 ng/mL, p = 0.02). CONCLUSION. Our results show that oral tolerization to CRA can improve the respiratory health of experimental mice in a CRA-induced model of asthma-like pulmonary inflammation by reducing pulmonary eosinophilia.en_US
dc.description.sponsorshipBoston University School of Medicine (Immunology Training Grant Program); National Institute of Environmental Health Sciences (5R01ES013538-04); National Institutes of Health (2T32AI007309-21A1)en_US
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.rightsCopyright 2010 Vaickus et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.titleOral Tolerance Inhibits Pulmonary Eosinophilia in a Cockroach Allergen Induced Model of Asthma: A Randomized Laboratory Studyen_US
dc.typearticleen_US
dc.identifier.doi10.1186/1465-9921-11-160en_US
dc.identifier.pubmedid21092270en_US
dc.identifier.pmcid3016351en_US


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