Tri-Nucleotide Receptors Play a Critical Role in Epithelial Cell Wound Repair


Show simple item record Weinger, Ilene en_US Klepeis, Veronica E. en_US Trinkaus-Randall, Vickery en_US 2012-01-11T21:40:13Z 2012-01-11T21:40:13Z 2005-7-29 en_US
dc.identifier.citation Weinger, Ilene, Veronica E. Klepeis, Vickery Trinkaus-Randall. "Tri-nucleotide receptors play a critical role in epithelial cell wound repair" Purinergic Signalling 1(3): 281-292. (2005) en_US
dc.identifier.issn 1573-9546 en_US
dc.description.abstract The cornea plays a major role in the refraction of light to the retina. Therefore, the integrity and transparency of the corneal epithelium are critical to vision. Following injury, a combination of rapid signal transduction events and long-term cell migration are essential for wound closure. We have demonstrated previously that injury resulted in the release of nucleotides that induce the propagation of a Ca2+ wave to neighboring cells. This suggests that nucleotides and their receptors are critical components of wound healing. Epidermal growth factor (EGF) and integrins also have been shown to play a role in injury. In this study, we demonstrate that pretreatment of cells with ATP and UTP inhibited the immediate wound response, while BzATP, ADP, and UDP did not affect this response. Tri-nucleotide pretreatment also reduced the EGF induced Ca2+ response. Additionally, lower EC50 concentrations of ATP and UTP triggered migration of cells that was enhanced further with EGF and was inhibited by the tripeptide, RGD. Results indicate that the desensitization induced by ATP and UTP was specific. While ADP and UDP cause a homologous desensitization of their own signal, they did not cause an inhibition of the wound response nor does BzATP. Neither Ca2+ wave propagation nor cell migration occurred in response to β,γ-MeATP. Together these results lead us to hypothesize that corneal epithelial wound repair is mediated by both P2Y2 and P2Y4 receptors. en_US
dc.description.sponsorship National Institutes of Health (EY06000); Massachusetts Lions Eye Research Fund; Research to Prevent Blindness, Inc.; New England Corneal Transplant Fund en_US
dc.language.iso en en_US
dc.publisher Springer Netherlands en_US
dc.rights Copyright Springer 2005 en_US
dc.subject Ca2+ imaging en_US
dc.subject Epidermal growth factor en_US
dc.subject Epithelium en_US
dc.subject Migration en_US
dc.subject Wound repair en_US
dc.title Tri-Nucleotide Receptors Play a Critical Role in Epithelial Cell Wound Repair en_US
dc.type article en_US
dc.identifier.doi 10.1007/s11302-005-8132-6 en_US
dc.identifier.pubmedid 18404512 en_US
dc.identifier.pmcid 2096543 en_US

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