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dc.contributor.authorWeinger, Ileneen_US
dc.contributor.authorKlepeis, Veronica E.en_US
dc.contributor.authorTrinkaus-Randall, Vickeryen_US
dc.date.accessioned2012-01-11T21:40:13Z
dc.date.available2012-01-11T21:40:13Z
dc.date.issued2005-7-29en_US
dc.identifier.citationWeinger, Ilene, Veronica E. Klepeis, Vickery Trinkaus-Randall. "Tri-nucleotide receptors play a critical role in epithelial cell wound repair" Purinergic Signalling 1(3): 281-292. (2005)en_US
dc.identifier.issn1573-9546en_US
dc.identifier.urihttp://hdl.handle.net/2144/3230
dc.description.abstractThe cornea plays a major role in the refraction of light to the retina. Therefore, the integrity and transparency of the corneal epithelium are critical to vision. Following injury, a combination of rapid signal transduction events and long-term cell migration are essential for wound closure. We have demonstrated previously that injury resulted in the release of nucleotides that induce the propagation of a Ca2+ wave to neighboring cells. This suggests that nucleotides and their receptors are critical components of wound healing. Epidermal growth factor (EGF) and integrins also have been shown to play a role in injury. In this study, we demonstrate that pretreatment of cells with ATP and UTP inhibited the immediate wound response, while BzATP, ADP, and UDP did not affect this response. Tri-nucleotide pretreatment also reduced the EGF induced Ca2+ response. Additionally, lower EC50 concentrations of ATP and UTP triggered migration of cells that was enhanced further with EGF and was inhibited by the tripeptide, RGD. Results indicate that the desensitization induced by ATP and UTP was specific. While ADP and UDP cause a homologous desensitization of their own signal, they did not cause an inhibition of the wound response nor does BzATP. Neither Ca2+ wave propagation nor cell migration occurred in response to β,γ-MeATP. Together these results lead us to hypothesize that corneal epithelial wound repair is mediated by both P2Y2 and P2Y4 receptors.en_US
dc.description.sponsorshipNational Institutes of Health (EY06000); Massachusetts Lions Eye Research Fund; Research to Prevent Blindness, Inc.; New England Corneal Transplant Funden_US
dc.language.isoenen_US
dc.publisherSpringer Netherlandsen_US
dc.rightsCopyright Springer 2005en_US
dc.subjectCa2+ imagingen_US
dc.subjectEpidermal growth factoren_US
dc.subjectEpitheliumen_US
dc.subjectMigrationen_US
dc.subjectWound repairen_US
dc.titleTri-Nucleotide Receptors Play a Critical Role in Epithelial Cell Wound Repairen_US
dc.typearticleen_US
dc.identifier.doi10.1007/s11302-005-8132-6en_US
dc.identifier.pubmedid18404512en_US
dc.identifier.pmcid2096543en_US


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