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dc.contributor.authorIlyinskii, Petr O.en_US
dc.contributor.authorGambaryan, Alexandra S.en_US
dc.contributor.authorMeriin, Anatoli B.en_US
dc.contributor.authorGabai, Vladimiren_US
dc.contributor.authorKartashov, Alexen_US
dc.contributor.authorThoidis, Galinien_US
dc.contributor.authorShneider, Alexander M.en_US
dc.date.accessioned2012-01-11T21:40:12Z
dc.date.available2012-01-11T21:40:12Z
dc.date.issued2008-1-16en_US
dc.identifier.citationIlyinskii, Petr O., Alexandra S. Gambaryan, Anatoli B. Meriin, Vladimir Gabai, Alex Kartashov, Galini Thoidis, Alexander M. Shneider. "Inhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiency" PLoS ONE3(1): 1417. (2008)en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/2144/3227
dc.description.abstractThe effectiveness of recombinant vaccines encoding full-length M2 protein of influenza virus or its ectodomain (M2e) have previously been tested in a number of models with varying degrees of success. Recently, we reported a strong cytotoxic effect exhibited by M2 on mammalian cells in vitro. Here we demonstrated a decrease in protection when M2 was added to a DNA vaccination regimen that included influenza NP. Furthermore, we have constructed several fusion proteins of conserved genes of influenza virus and tested their expression in vitro and protective potential in vivo. The four-partite NP-M1-M2-NS1 fusion antigen that has M2 sequence engineered in the middle part of the composite protein was shown to not be cytotoxic in vitro. A three-partite fusion protein (consisting of NP, M1 and NS1) was expressed much more efficiently than the four-partite protein. Both of these constructs provided statistically significant protection upon DNA vaccination, with construct NP-M1-M2-NS1 being the most effective. We conclude that incorporation of M2 into a vaccination regimen may be beneficial only when its apparent cytotoxicity-linked negative effects are neutralized. The possible significance of this data for influenza vaccination regimens and preparations is discussed.en_US
dc.description.sponsorshipCure Lab, Inc.en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.titleInhibition of Influenza M2-Induced Cell Death Alleviates Its Negative Contribution to Vaccination Efficiencyen_US
dc.typearticleen_US
dc.identifier.doi10.1371/journal.pone.0001417en_US
dc.identifier.pubmedid18197240en_US
dc.identifier.pmcid2175529en_US


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