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dc.contributor.authorLiu, Donglinen_US
dc.contributor.authorBrockman, J. Michaelen_US
dc.contributor.authorDass, Brindaen_US
dc.contributor.authorHutchins, Lucie N.en_US
dc.contributor.authorSingh, Priyamen_US
dc.contributor.authorMcCarrey, John R.en_US
dc.contributor.authorMacDonald, Clinton C.en_US
dc.contributor.authorGraber, Joel H.en_US
dc.date.accessioned2012-01-11T21:10:57Z
dc.date.available2012-01-11T21:10:57Z
dc.date.issued2006-12-08en_US
dc.identifier.citationLiu, Donglin, J. Michael Brockman, Brinda Dass, Lucie N. Hutchins, Priyam Singh, John R. McCarrey, Clinton C. MacDonald, Joel H. Graber. "Systematic variation in mRNA 3′-processing signals during mouse spermatogenesis" Nucleic Acids Research 35(1): 234-246. (2006)en_US
dc.identifier.issn1362-4962en_US
dc.identifier.urihttp://hdl.handle.net/2144/3205
dc.description.abstractGene expression and processing during mouse male germ cell maturation (spermatogenesis) is highly specialized. Previous reports have suggested that there is a high incidence of alternative 3′-processing in male germ cell mRNAs, including reduced usage of the canonical polyadenylation signal, AAUAAA. We used EST libraries generated from mouse testicular cells to identify 3′-processing sites used at various stages of spermatogenesis (spermatogonia, spermatocytes and round spermatids) and testicular somatic Sertoli cells. We assessed differences in 3′-processing characteristics in the testicular samples, compared to control sets of widely used 3′-processing sites. Using a new method for comparison of degenerate regulatory elements between sequence samples, we identified significant changes in the use of putative 3′-processing regulatory sequence elements in all spermatogenic cell types. In addition, we observed a trend towards truncated 3′-untranslated regions (3′-UTRs), with the most significant differences apparent in round spermatids. In contrast, Sertoli cells displayed a much smaller trend towards 3′-UTR truncation and no significant difference in 3′-processing regulatory sequences. Finally, we identified a number of genes encoding mRNAs that were specifically subject to alternative 3′-processing during meiosis and postmeiotic development. Our results highlight developmental differences in polyadenylation site choice and in the elements that likely control them during spermatogenesis.en_US
dc.description.sponsorshipNational Institutes of Health (NCRR INBRE Maine 2 P20 RR16463, NIGMS GM072706, NICHD HD037102)en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.titleSystematic Variation in mRNA 3′-Processing Signals during Mouse Spermatogenesisen_US
dc.typearticleen_US
dc.identifier.doi10.1093/nar/gkl919en_US
dc.identifier.pubmedid17158511en_US
dc.identifier.pmcid1802579en_US


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