Determinants of Leukocyte Margination in Rectangular Microchannels


Show simple item record Jain, Abhishek en_US Munn, Lance L. en_US 2012-01-11T00:42:54Z 2012-01-11T00:42:54Z 2009-9-21 en_US
dc.identifier.citation Jain, Abhishek, Lance L. Munn. "Determinants of Leukocyte Margination in Rectangular Microchannels" PLoS ONE 4(9): e7104. (2009) en_US
dc.identifier.issn 1932-6203 en_US
dc.description.abstract Microfabrication of polydimethylsiloxane (PDMS) devices has provided a new set of tools for studying fluid dynamics of blood at the scale of real microvessels. However, we are only starting to understand the power and limitations of this technology. To determine the applicability of PDMS microchannels for blood flow analysis, we studied white blood cell (WBC) margination in channels of various geometries and blood compositions. We found that WBCs prefer to marginate downstream of sudden expansions, and that red blood cell (RBC) aggregation facilitates the process. In contrast to tubes, WBC margination was restricted to the sidewalls in our low aspect ratio, pseudo-2D rectangular channels and consequently, margination efficiencies of more than 95% were achieved in a variety of channel geometries. In these pseudo-2D channels blood rheology and cell integrity were preserved over a range of flow rates, with the upper range limited by the shear in the vertical direction. We conclude that, with certain limitations, rectangular PDMS microfluidic channels are useful tools for quantitative studies of blood rheology. en_US
dc.description.sponsorship National Institutes of Health (R21 CA126761) en_US
dc.language.iso en en_US
dc.publisher Public Library of Science en_US
dc.rights Jain et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_US
dc.title Determinants of Leukocyte Margination in Rectangular Microchannels en_US
dc.type article en_US
dc.identifier.doi 10.1371/journal.pone.0007104 en_US
dc.identifier.pubmedid 19768109 en_US
dc.identifier.pmcid 2740820 en_US

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