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dc.contributor.authorRachlin, Johnen_US
dc.contributor.authorDing, Chunmingen_US
dc.contributor.authorCantor, Charlesen_US
dc.contributor.authorKasif, Simonen_US
dc.date.accessioned2012-01-11T00:39:15Z
dc.date.available2012-01-11T00:39:15Z
dc.date.issued2005-06-27en_US
dc.identifier.citationRachlin, John, Chunming Ding, Charles Cantor, Simon Kasif. "MuPlex: multi-objective multiplex PCR assay design" Nucleic Acids Research 33(Web Server issue): W544-W547. (2005)en_US
dc.identifier.issn1362-4962en_US
dc.identifier.urihttp://hdl.handle.net/2144/3023
dc.description.abstractWe have developed a web-enabled system called MuPlex that aids researchers in the design of multiplex PCR assays. Multiplex PCR is a key technology for an endless list of applications, including detecting infectious microorganisms, whole-genome sequencing and closure, forensic analysis and for enabling flexible yet low-cost genotyping. However, the design of a multiplex PCR assays is computationally challenging because it involves tradeoffs among competing objectives, and extensive computational analysis is required in order to screen out primer-pair cross interactions. With MuPlex, users specify a set of DNA sequences along with primer selection criteria, interaction parameters and the target multiplexing level. MuPlex designs a set of multiplex PCR assays designed to cover as many of the input sequences as possible. MuPlex provides multiple solution alternatives that reveal tradeoffs among competing objectives. MuPlex is uniquely designed for large-scale multiplex PCR assay design in an automated high-throughput environment, where high coverage of potentially thousands of single nucleotide polymorphisms is required. The server is available at http://genomics14.bu.edu:8080/MuPlex/MuPlex.html.en_US
dc.description.sponsorshipNational Science Foundation (DBI-0239435, ITR-048715); National Human Genome Research Institute (1R33HG002850-01A1)en_US
dc.language.isoenen_US
dc.publisherOxford University Pressen_US
dc.titleMuPlex: Multi-Objective Multiplex PCR Assay Designen_US
dc.typearticleen_US
dc.identifier.doi10.1093/nar/gki377en_US
dc.identifier.pubmedid15980531en_US
dc.identifier.pmcid1160138en_US


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