Show simple item record

dc.contributor.authorChiavolini, Damianaen_US
dc.contributor.authorRangel-Moreno, Javieren_US
dc.contributor.authorBerg, Gretchenen_US
dc.contributor.authorChristian, Kateen_US
dc.contributor.authorOliveira-Nascimento, Lauraen_US
dc.contributor.authorWeir, Susanen_US
dc.contributor.authorAlroy, Josephen_US
dc.contributor.authorRandall, Troy D.en_US
dc.contributor.authorWetzler, Lee M.en_US
dc.date.accessioned2012-01-09T21:00:24Z
dc.date.available2012-01-09T21:00:24Z
dc.date.issued2010-6-16en_US
dc.identifier.citationChiavolini, Damiana, Javier Rangel-Moreno, Gretchen Berg, Kate Christian, Laura Oliveira-Nascimento, Susan Weir, Joseph Alroy, Troy D. Randall, Lee M. Wetzler. "Bronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemia" PLoS ONE 5(6): e11156. (2010)en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/2144/2980
dc.description.abstractBACKGROUND. Francisella tularensis causes severe pulmonary disease, and nasal vaccination could be the ideal measure to effectively prevent it. Nevertheless, the efficacy of this type of vaccine is influenced by the lack of an effective mucosal adjuvant. METHODOLOGY/PRINCIPAL FINDINGS. Mice were immunized via the nasal route with lipopolysaccharide isolated from F. tularensis and neisserial recombinant PorB as an adjuvant candidate. Then, mice were challenged via the same route with the F. tularensis attenuated live vaccine strain (LVS). Mouse survival and analysis of a number of immune parameters were conducted following intranasal challenge. Vaccination induced a systemic antibody response and 70% of mice were protected from challenge as showed by their improved survival and weight regain. Lungs from mice recovering from infection presented prominent lymphoid aggregates in peribronchial and perivascular areas, consistent with the location of bronchus-associated lymphoid tissue (BALT). BALT areas contained proliferating B and T cells, germinal centers, T cell infiltrates, dendritic cells (DCs). We also observed local production of antibody generating cells and homeostatic chemokines in BALT areas. CONCLUSIONS. These data indicate that PorB might be an optimal adjuvant candidate for improving the protective effect of F. tularensis antigens. The presence of BALT induced after intranasal challenge in vaccinated mice might play a role in regulation of local immunity and long-term protection, but more work is needed to elucidate mechanisms that lead to its formation.en_US
dc.description.sponsorshipNational Institute of Allergy and Infectious Diseases (U19 AI056543); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Conselho Nacional de Desenvolvimento Científico e Tecnológicoen_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.rightsChiavolini et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.titleBronchus-Associated Lymphoid Tissue (BALT) and Survival in a Vaccine Mouse Model of Tularemiaen_US
dc.typearticleen_US
dc.identifier.doi10.1371/journal.pone.0011156en_US
dc.identifier.pubmedid20585390en_US
dc.identifier.pmcid2886834en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record