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dc.contributor.authorWright, Robert O.en_US
dc.contributor.authorSchwartz, Joelen_US
dc.contributor.authorWright, Rosalind J.en_US
dc.contributor.authorBollati, Valentinaen_US
dc.contributor.authorTarantini, Letiziaen_US
dc.contributor.authorPark, Sung Kyunen_US
dc.contributor.authorHu, Howarden_US
dc.contributor.authorSparrow, Daviden_US
dc.contributor.authorVokonas, Pantelen_US
dc.contributor.authorBaccarelli, Andreaen_US
dc.date.accessioned2012-01-09T14:20:00Z
dc.date.available2012-01-09T14:20:00Z
dc.date.issued2010-06en_US
dc.identifier.citationWright, Robert O., Joel Schwartz, Rosalind J. Wright, Valentina Bollati, Letizia Tarantini, Sung Kyun Park, Howard Hu, David Sparrow, Pantel Vokonas, Andrea Baccarelli. "Biomarkers of Lead Exposure and DNA Methylation within Retrotransposons" Environmental Health Perspectives 118(6): 790-795. (2010)en_US
dc.identifier.issn1552-9924en_US
dc.identifier.urihttp://hdl.handle.net/2144/2753
dc.description.abstractBACKGROUND. DNA methylation is an epigenetic mark that regulates gene expression. Changes in DNA methylation within white blood cells may result from cumulative exposure to environmental metals such as lead. Bone lead, a marker of cumulative exposure, may therefore better predict DNA methylation than does blood lead. OBJECTIVE. In this study we compared associations between lead biomarkers and DNA methylation. METHODS. We measured global methylation in participants of the Normative Aging Study (all men) who had archived DNA samples. We measured patella and tibia lead levels by K-X-Ray fluorescence and blood lead by atomic absorption spectrophotometry. DNA samples from blood were used to determine global methylation averages within CpG islands of long interspersed nuclear elements-1 (LINE-1) and Alu retrotransposons. A mixed-effects model using repeated measures of Alu or LINE-1 as the dependent variable and blood/bone lead (tibia or patella in separate models) as the primary exposure marker was fit to the data. RESULTS. Overall mean global methylation (± SD) was 26.3 ± 1.0 as measured by Alu and 76.8 ± 1.9 as measured by LINE-1. In the mixed-effects model, patella lead levels were inversely associated with LINE-1 (β = -0.25; p < 0.01) but not Alu (β = -0.03; p = 0.4). Tibia lead and blood lead did not predict global methylation for either Alu or LINE-1. CONCLUSION. Patella lead levels predicted reduced global DNA methylation within LINE-1 elements. The association between lead exposure and LINE-1 DNA methylation may have implications for the mechanisms of action of lead on health outcomes, and also suggests that changes in DNA methylation may represent a biomarker of past lead exposure.en_US
dc.description.sponsorshipUnited States Department of Veterans Affairs; Massachusetts Veterans Epidemiology Research and Information Center; National Institutes of Health (R01ES015172, ES014663, R01ES005257, R01ES013744, K23ES000381, P30ES00002); Leaves of Grass Foundationen_US
dc.language.isoenen_US
dc.publisherNational Institute of Environmental Health Sciencesen_US
dc.rightsThis is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI.en_US
dc.subjectAgingen_US
dc.subjectDNA methylationen_US
dc.subjectEpigeneticsen_US
dc.subjectLeaden_US
dc.subjectMetalsen_US
dc.titleBiomarkers of Lead Exposure and DNA Methylation within Retrotransposonsen_US
dc.typearticleen_US
dc.identifier.doi10.1289/ehp.0901429en_US
dc.identifier.pubmedid20064768en_US
dc.identifier.pmcid2898855en_US


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