Biomarkers of Lead Exposure and DNA Methylation within Retrotransposons


Show simple item record Wright, Robert O. en_US Schwartz, Joel en_US Wright, Rosalind J. en_US Bollati, Valentina en_US Tarantini, Letizia en_US Park, Sung Kyun en_US Hu, Howard en_US Sparrow, David en_US Vokonas, Pantel en_US Baccarelli, Andrea en_US 2012-01-09T14:20:00Z 2012-01-09T14:20:00Z 2010-06 en_US
dc.identifier.citation Wright, Robert O., Joel Schwartz, Rosalind J. Wright, Valentina Bollati, Letizia Tarantini, Sung Kyun Park, Howard Hu, David Sparrow, Pantel Vokonas, Andrea Baccarelli. "Biomarkers of Lead Exposure and DNA Methylation within Retrotransposons" Environmental Health Perspectives 118(6): 790-795. (2010) en_US
dc.identifier.issn 1552-9924 en_US
dc.description.abstract BACKGROUND. DNA methylation is an epigenetic mark that regulates gene expression. Changes in DNA methylation within white blood cells may result from cumulative exposure to environmental metals such as lead. Bone lead, a marker of cumulative exposure, may therefore better predict DNA methylation than does blood lead. OBJECTIVE. In this study we compared associations between lead biomarkers and DNA methylation. METHODS. We measured global methylation in participants of the Normative Aging Study (all men) who had archived DNA samples. We measured patella and tibia lead levels by K-X-Ray fluorescence and blood lead by atomic absorption spectrophotometry. DNA samples from blood were used to determine global methylation averages within CpG islands of long interspersed nuclear elements-1 (LINE-1) and Alu retrotransposons. A mixed-effects model using repeated measures of Alu or LINE-1 as the dependent variable and blood/bone lead (tibia or patella in separate models) as the primary exposure marker was fit to the data. RESULTS. Overall mean global methylation (± SD) was 26.3 ± 1.0 as measured by Alu and 76.8 ± 1.9 as measured by LINE-1. In the mixed-effects model, patella lead levels were inversely associated with LINE-1 (β = -0.25; p < 0.01) but not Alu (β = -0.03; p = 0.4). Tibia lead and blood lead did not predict global methylation for either Alu or LINE-1. CONCLUSION. Patella lead levels predicted reduced global DNA methylation within LINE-1 elements. The association between lead exposure and LINE-1 DNA methylation may have implications for the mechanisms of action of lead on health outcomes, and also suggests that changes in DNA methylation may represent a biomarker of past lead exposure. en_US
dc.description.sponsorship United States Department of Veterans Affairs; Massachusetts Veterans Epidemiology Research and Information Center; National Institutes of Health (R01ES015172, ES014663, R01ES005257, R01ES013744, K23ES000381, P30ES00002); Leaves of Grass Foundation en_US
dc.language.iso en en_US
dc.publisher National Institute of Environmental Health Sciences en_US
dc.rights This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original DOI. en_US
dc.subject Aging en_US
dc.subject DNA methylation en_US
dc.subject Epigenetics en_US
dc.subject Lead en_US
dc.subject Metals en_US
dc.title Biomarkers of Lead Exposure and DNA Methylation within Retrotransposons en_US
dc.type article en_US
dc.identifier.doi 10.1289/ehp.0901429 en_US
dc.identifier.pubmedid 20064768 en_US
dc.identifier.pmcid 2898855 en_US

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