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dc.contributor.authorSridhar, Sriramen_US
dc.contributor.authorSchembri, Franken_US
dc.contributor.authorZeskind, Julieen_US
dc.contributor.authorShah, Vishalen_US
dc.contributor.authorGustafson, Adam M.en_US
dc.contributor.authorSteiling, Katrinaen_US
dc.contributor.authorLiu, Gangen_US
dc.contributor.authorDumas, Yves-Martineen_US
dc.contributor.authorZhang, Xiaohuien_US
dc.contributor.authorBrody, Jerome S.en_US
dc.contributor.authorLenburg, Marc E.en_US
dc.contributor.authorSpira, Avrumen_US
dc.date.accessioned2011-12-29T23:59:38Z
dc.date.available2011-12-29T23:59:38Z
dc.date.copyright2008
dc.date.issued2008-5-30
dc.identifier.citationSridhar, Sriram, Frank Schembri, Julie Zeskind, Vishal Shah, Adam M Gustafson, Katrina Steiling, Gang Liu, Yves-Martine Dumas, Xiaohui Zhang, Jerome S Brody, Marc E Lenburg, Avrum Spira. "Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium." BMC Genomics 9:259. (2008)
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/2144/2671
dc.description.abstractBACKGROUND: Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We have previously characterized gene expression in the bronchial epithelium of never smokers and identified the gene expression changes that occur in the mainstem bronchus in response to smoking. In this study, we explored relationships in whole-genome gene expression between extrathorcic (buccal and nasal) and intrathoracic (bronchial) epithelium in healthy current and never smokers. RESULTS: Using genes that have been previously defined as being expressed in the bronchial airway of never smokers (the "normal airway transcriptome"), we found that bronchial and nasal epithelium from non-smokers were most similar in gene expression whencompared to other epithelial and nonepithelial tissues, with several antioxidant, detoxification, and structural genes being highly expressed in both the bronchus and nose. Principle component analysis of previously defined smoking-induced genes from the bronchus suggested that smoking had a similar effect on gene expression in nasal epithelium. Gene set enrichment analysis demonstrated that this set of genes was also highly enriched among the genes most altered by smoking in both nasal and buccal epithelial samples. The expression of several detoxification genes was commonly altered by smoking in all three respiratory epithelial tissues, suggesting a common airway-wide response to tobacco exposure. CONCLUSION: Our findings support a relationship between gene expression in extra- and intrathoracic airway epithelial cells and extend the concept of a smoking-induced field of injury to epithelial cells that line the mouth and nose. This relationship could potentially be utilized to develop a non-invasive biomarker for tobacco exposure as well as a non-invasive screening or diagnostic tool providing information about individual susceptibility to smoking-induced lung diseases.en_US
dc.description.sponsorshipDoris Duke Charitable Foundation; National Institute of Health and National Cancer Institute (R01CA124640); National Institute of Health and National Institute of Environmental Health Sciences (U01ES016035)en_US
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.rightsCopyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.titleSmoking-Induced Gene Expression Changes in the Bronchial Airway Are Reflected in Nasal and Buccal Epitheliumen_US
dc.typearticleen_US
dc.identifier.doi10.1186/1471-2164-9-259
dc.identifier.pubmedid18513428
dc.identifier.pmcid2435556


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Copyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as Copyright 2008 Sridhar et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution 2.0 License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.