Show simple item record

dc.contributor.authorGao, Yangen_US
dc.contributor.authorWolf, Lauren K.en_US
dc.contributor.authorGeorgiadis, Rosina M.en_US
dc.date.accessioned2009-04-13T23:23:26Z
dc.date.available2009-04-13T23:23:26Z
dc.date.issued2006en_US
dc.identifier.citation2006. "Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison," Nucleic Acids Research. vol. 34 issue. 11 .en_US
dc.identifier.otherPMC1488884en_US
dc.identifier.uri10.1093/nar/gkl422en_US
dc.identifier.urihttp://hdl.handle.net/2144/1006
dc.description.abstractThe hybridization kinetics for a series of designed 25mer probe�target pairs having varying degrees of secondary structure have been measured by UV absorbance and surface plasmon resonance (SPR) spectroscopy in solution and on the surface, respectively. Kinetic rate constants derived from the resultant data decrease with increasing probe and target secondary structure similarly in both solution and surface environments. Specifically, addition of three intramolecular base pairs in the probe and target structure slow hybridization by a factor of two. For individual strands containing four or more intramolecular base pairs, hybridization cannot be described by a traditional two-state model in solution-phase nor on the surface. Surface hybridization rates are also 20- to 40-fold slower than solution-phase rates for identical sequences and conditions. These quantitative findings may have implications for the design of better biosensors, particularly those using probes with deliberate secondary structure.en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.relation.ispartofseriesvol. 34 issue. 11en_US
dc.titleSecondary structure effects on DNA hybridization kinetics: a solution versus surface comparisonen_US
dc.typearticleen_US


Files in this item

This item appears in the following Collection(s)

Show simple item record